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The expression of ATP6V1H induced by OM was decreased after HG treatment. (A-C) The mRNA and protein expression levels of ATP6V1H significantly increased 14 days after OM treatment. (D-F) HG treatment rescued the upregulation of ATP6V1H induced by OM at day 14. OM, osteogenic medium; HG, high glucose-free fatty acids. Control, MC3T3-E1 cells cultured in α-MEM with 10% FBS for 14 days. * P < .01, compared with the control group; # P < .01, compared with OM group.
