Skip to main content
. 2019 Jul 4;15(2):43–54. doi: 10.1080/15476278.2019.1633869

FIGURE 3.

FIGURE 3.

ATP6V1H overexpression facilitates osteogenic differentiation. (A-C) ATP6V1H was overexpressed by transfection of plasmid ATP6V1H. (D-F) Runx2 expression was restored by ATP6V1H compared to combined treatments with OM and HG. (G-I) Col1α1 expression was restored by ATP6V1H compared to combined treatments with OM and HG. (J) ALP activity ALP was restored by ATP6V1H compared to combined treatments with OM and HG. (K) The Alizarin Red staining was performed to determine the mineralized degree and (L) the absorbance showed quantified results. OM, osteogenic medium; HG, high glucose-free fatty acids; p-NC, negative control plasmid; p-ATP6V1H, ATP6V1H plasmid. *P < .01, compared with the control group; #P < .01, compared with OM group.