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. 2019 Jul 4;15(2):43–54. doi: 10.1080/15476278.2019.1633869

FIGURE 5.

FIGURE 5.

Knockdown of ATP6V1H upregulates the Akt/GSK3β signaling pathway in MC3T3-E1 cells. (A) The expression of ATP6V1H was detected by western blotting of protein samples from high glucose cultures of MC3T3-E1 cells transfected with si-NC or si-ATP6V1H. (B) Quantitative assay of ATP6V1H western blot was performed by Image J software. (C) The expression of p-Akt, Akt, p-GSK3β and GSK3β was detected by western blotting of protein samples from high glucose cultures of MC3T3-E1 cells transfected with si-NC or si-ATP6V1H. (D) Quantitative assay was performed by Image J software. OM, osteogenic medium; HG, high glucose-free fatty acids; sh-NC, negative control siRNA; si-ATP6V1H, ATP6V1H siRNA; Akt, Serine/Threonine Kinase 1; GSK-3β, Glycogen Synthase Kinase 3 beta. *P < .01, compared with OM+HG+si-NC group.