Skip to main content
. 2019 Jul 2;129(8):3153–3170. doi: 10.1172/JCI125170

Figure 3. Pharmacological Smo inhibition exacerbates AD in WT mice.

Figure 3

Data from 2 independent experiments with 7 Oxa-treated WT mice per group unless otherwise stated. Control (black, DMSO-only–injected) and Smo-inh–injected (red) mice; each symbol represents an individual animal. (A) Scheme of Oxa administration with i.p. Smo-inh or DMSO injection. (B) Plot shows ear thickness from Oxa-treated control and Smo-inh–injected groups. (C) Representative H&E images of ear sections from Oxa-treated WT mice injected either with DMSO only (left image) or Smo-inh (right) at termination (day 14). Scale bar: 100 μm. Plots show dermal and epidermal thickness of Oxa-treated WT without Smo-inh (DMSO-only control) and with Smo-inh on day 14. (D) Serum IgE concentration from Oxa-treated control and Smo-inh–injected mice measured by ELISA. (E) Shh expression (QRT-PCR) in whole ear homogenates from Oxa-treated and Oxa-untreated mice without Smo-inh injection (DMSO-only control) and with Smo-inh injection. (F) Percentage and number of skin leukocytes (CD45+), macrophages (Mϕ; CD45+CD11b+F4/80+) and eosinophils (Eos; CD45+CD11b+SiglecF+) from ear skin from Oxa-treated control (DMSO only) and Smo-inh–injected mice. (G) Percentage of skin CD4+ T cells that expressed IFN-γ and IL-17 from Oxa-treated control (DMSO only) and Smo-inh–injected mice. (H) Percentage of skin CD8+ T cells that expressed IFN-γ and IL-17 from Oxa-treated control (without Smo-inh, DMSO only) and Smo-inh–injected mice. (I) Percentage of skin CD4+ T cells that are Treg (CD3+CD25+icFoxp3+) from Oxa-treated control (without Smo-inh, DMSO only) and Smo-inh–injected mice. Plots are mean ± SEM; 2-tailed unpaired Student’s t test. *P < 0.05, **P < 0.01, and ***P < 0.001.