Skip to main content
. 2019 Jun 24;129(8):3058–3071. doi: 10.1172/JCI122936

Figure 7. Ctsk silencing in Ocy454 cells affects the expression of genes involved in bone remodeling.

Figure 7

(A) Efficiency of Ctsk deletion in Ocy454 cells. Control shRNA (black dots) and Ctsk shRNA (red dots) (catalog TRCN0000054624). Data shown represent the mean of triplicate experiments ± SEM and were normalized to the expression of 18S mRNA (n = 3). *P < 0.05 by Student’s 2-tailed t test. (B) Densitometric analysis of Western blotting showing the efficiency of Ctsk silencing. β-Tubulin was used as a loading control (n = 3). (C) Ctsk immunostaining in control shRNA and Ctsk shRNA Ocy454 cells showing Ctsk (red), actin (green) and nucleus (blue) (n = 3). Scale bars: 20 μm. (D) qRT-PCR analysis of mRNA expression in control shRNA (black dots) and Ctsk shRNA (red dots) Ocy454 cells. Data shown are the mean of triplicate experiments ± SEM and were normalized to the expression of 18S mRNA (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001, by Student’s t test for comparisons of control shRNA with Ctsk shRNA at each time point.