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. 2019 Jul 22;129(8):3448–3463. doi: 10.1172/JCI96273

Figure 8. Partial knockdown of CFTR in NhBE cells decreases VX809-mediated activation of Nrf2.

Figure 8

Primary NhBE cells were infected with CFTR shRNA or scrambled control (Scr Con) lentivirus for 4 days, then treated with DMSO control or 1–10 μM VX809 for 48 hours. (A) Gene expression of CFTR was determined by real-time qPCR, with mRNA levels expressed as fold changes versus Scr Con with vehicle control (DMSO). (B–D) Gene expression of Nrf2 target genes (HMOX1, NQO1, or GCLC) was determined by qPCR, with mRNA levels shown as fold changes versus Scr Con or CFTR shRNA with vehicle control (DMSO), respectively. Data for 3 independent experiments on 3 non-CF donors with 4 replicates per treatment per donor are expressed as box-and-whisker plots. Horizontal bars indicate the median, box borders indicate 25th and 75th percentiles, and whiskers indicate 5th and 95th percentiles. *P < 0.05 and **P < 0.01 vs. no drug control, while #P < 0.05 vs. same drug concentration scrambled shRNA control by mixed-effects ANOVA with Dunnett’s multiple-comparisons test.