Figure 5. Evaluation of the magnetic hyperthermia (MHT). In vitro (I-IV assays were conducted, comparing to C6 cells alone (I), C6 + MHT (II), and C6 + aminosilane-coated superparamagnetic iron oxide nanoparticles (SPIONsAmin) (III), and C6 + SPIONsAmin + MHT (IV), showed a 52% reduction in the intensity of bioluminescence in C6 cells marked with SPIONsAmin, and submitted to MHT. There was no significant difference between conditions (I), (II), and (III). In vivo assays (A-D) were performed, where in (A), we noted that bioluminescence in and of itself, without the application of luciferin, does not interfere in the capture of the bioluminescence signal. In (B), the tumor that developed 21 days after the tumor induction issued a bioluminescence relative to which, after (C) the application of MHT (200 Gauss, 874kHz) for 40 minutes, (D) a reduction of 32.8% was observed.
