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. 2019 Jul 31;476(14):2111–2125. doi: 10.1042/BCJ20190154

Figure 3. CO binding to BvHbs by flash photolysis.

Figure 3.

(A) Time courses for rebinding of CO to BvHb1.2, after flash photolysis, monitored at 416 nm. The binding is a double exponential process at all CO concentrations (from left to right: 500, 250, 125, and 63 µM). (B) Fast phase and (C) Slow phase of the rebinding of CO to the three BvHbs. For BvHb1.1 and BvHb1.2 (both class-1) the approximate rates of the slow phase are essentially the same as reported by stopped-flow (Figure 2B). For BvHb2, this slow phase is faster than the value given by stopped-flow spectrometry. The closed and open symbols correspond to kobs values monitored at 429 nm for BvHb2 and 416 nm and 430 nm for BvHb1.1 and BvHb1.2.