Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 31;15(7):e1008240. doi: 10.1371/journal.pgen.1008240

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Pajak et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 3 — (A,B) Poly(A) tail length in individually sequenced clones after 3′RACE analysis of ND1 transcripts in dmpnpase^KD, dmpnpase^KD/dmsuv3^KD, dmsuv3^OE, dmpnpase^OE, dmpnpase^OE/dmsuv3^OE, and control (w;;) larvae at 4 days AEL. (C) Quantification of mitochondrial mRNA steady-state levels of dmpnpase^OE (dmmtpap^KO/FM7;;UAS-dmpnpase/daGAL4 or FM7;;UAS-dmpnpase/daGAL4 or FM7/Y;;UAS-dmpnpase/daGAL4), dmmtpap^KO (dmmtpap^KO/Y;;) dmpnpase^OE/dmmtpap^KO (dmmtpap^KO/ Y;;UAS-dmpnpase/daGAL4), and control (w;;) larvae at 4 days AEL. Histone was used as loading control. (D) Mitochondrial mRNA steady-state levels in dmlrpprc1^KD, dmlrpprc1^KD/dmsuv3^KD, dmlrpprc1^KD/dmpnpase^KD, and control (w;;) larvae at 4 days AEL, as determined by qRT-PCR. RP49 was used as an endogenous control. (E) 3′RACE analysis of poly(A) tails of ND1 transcripts in dmlrpprc1^KD (w;;UAS-bsfRNAi#1/daGAL4), dmlrpprc1^KD/dmsuv3^KD (w;UAS-dmsuv3RNAi/+;UAS-bsfRNAi#1/daGAL4), dmlrpprc1^KD/ dmpnpase^KD (w;UAS-dmpnpaseRNAi/+;UAS-bsfRNAi#1/daGAL4), and control (w;;) larvae at 4 days AEL. All data are represented as mean +/- SEM (***p < 0.001, **p< 0.01, *p< 0.05, n = 5).