Fig 4. Mitochondrial dRNAs accumulate and localise to the cytoplasm in the absence of mtPNPase, SUV3 and mtPAP.

(A) Northern blot analysis by formaldehyde-agarose gel electrophoresis with total RNA isolated from 4 day AEL larvae. The signals were detected using double stranded DNA probes and antisense strand oligonucleotide probes. Nuclear encoded RP49 was used as a loading control. (B) Poly(A) tail length of COX1 transcripts and COX1 antisense strands in individually sequenced clones after 3′RACE experiments in dmpnpase^KD (n = 22 and n = 31, respectively), and wild type control (w;;, n = 23 and n = 5, respectively) larvae at 4 days AEL. (C) Northern blots of total RNA isolated from larvae at 4 days AEL of controls, dmsuv3^KD, dmpnpase^KO, dmmtpap^KO, and dmlrpprc1^KD treated with different RNases as indicated. The blots were probed with 5 different probes against mitochondrial transcripts and with RP49 as a loading control. (D) Immunostaining of dsRNA with J2 antibodies (red) in dissected brains from larvae of control, dmpnpase^KO, dmsuv3^KD, dmmtpap^KO, and dmlrpprc1^KD. Mitochondria are stained with ATP5a antibodies (green) and nuclei are stained with DAPI (blue).