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. 2019 Jul 31;14(7):e0220333. doi: 10.1371/journal.pone.0220333

Fig 2. ESL thickness is reduced in Ext1+/-Ext2+/- mice and increased by high sodium diet.

Fig 2

(A) Control mice had a larger ESL (NSD: 0.18±0.04 μm, n = 6; HSD: 0.32±0.05 μm, n = 3) than Ext1+/-Ext2+/- mice on both diets (NSD: 0.04±0.04 μm, n = 6, p = 0.04; HSD 0.07±0.03μm, n = 7, p = 0.004). There was no difference in ESL thickness between diets within control mice (p = 0.07) or Ext1+/-Ext2+/- mice (p = 0.47). (B) In a subset of 5–20 μm vessels with a mean diameter of 12.7±0.8 μm, control (0.07±0.03μm, n = 4) and Ext1+/-Ext2+/- mice (0.04±0.02 μm, n = 5) had equal ESL thickness on a NSD (p = 0.38). On a HSD, control mice (0.24±0.01 μm, n = 3) had a thicker ESL than Ext1+/-Ext2+/- mice (0.02±0.04 μm, n = 7, p = 0.005). ESL thickness was not affected by diet within control mice (p = 0.06) or Ext1+/-Ext2+/- mice (p = 1.00). (C) In 20–40 μm vessels with a mean diameter of 28.6±0.4 μm, controls (NSD: 0.24±0.03 μm, n = 6; HSD 0.36±0.05 μm, n = 3) had a significant larger ESL than Ext1+/-Ext2+/- mice on both diets (NSD: 0.04±0.02 μm, n = 6, p = 0.02; HSD: 0.11±0.05μm, n = 7, p = 0.02). HSD increased ESL thickness in control mice (p = 0.02), but not in Ext1+/-Ext2+/- mice (p = 0.53). *p<0.05. Con, controls; ESL, endothelial surface layer; Ext, Ext1+/-Ext2+/-; HSD, high sodium diet; NSD, normal sodium diet.