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. 2019 Jul 31;14(7):e0220671. doi: 10.1371/journal.pone.0220671

Table 1. Primer pairs used to amplify the genes studied by real-time RT-PCR.

Target gene 5′primer 3′primer Source
rrs GGGAGACTTGAGTGCAGGAG GTGCCTCAGCGTCAGTTACA [43]
spoA CTCAAAGCGCAATAAATCTAGGAGC TTGAGTCTCTTGAACTGGTCTAGG [44]
sigH GTTGGTAGCAAAAGAAAAAAGTTATGAG GTACTCTAGTGCTATTTTATCCCCTTCAC [45]
slpA AATGATAAAGCATTTGTAGTTGGTG TATTGGAGTAGCATCTCCATC [43]
cwp84 TGGGCAACTGGTGGAAAATA TAGTTGCACCTTGTGCCTCA [43]
luxS GTGTACTTGATGGAGTAAAGGGAGA TTCTACATCCCATTGGAGATAAGTC [46]
agrD1 TTTGCTAGCTCATTGGCACTT GATTGCTGATTTCTTTGGGTACTT Primer3 software