Table 1. Primer pairs used to amplify the genes studied by real-time RT-PCR.
Target gene | 5′primer | 3′primer | Source |
---|---|---|---|
rrs | GGGAGACTTGAGTGCAGGAG | GTGCCTCAGCGTCAGTTACA | [43] |
spoA | CTCAAAGCGCAATAAATCTAGGAGC | TTGAGTCTCTTGAACTGGTCTAGG | [44] |
sigH | GTTGGTAGCAAAAGAAAAAAGTTATGAG | GTACTCTAGTGCTATTTTATCCCCTTCAC | [45] |
slpA | AATGATAAAGCATTTGTAGTTGGTG | TATTGGAGTAGCATCTCCATC | [43] |
cwp84 | TGGGCAACTGGTGGAAAATA | TAGTTGCACCTTGTGCCTCA | [43] |
luxS | GTGTACTTGATGGAGTAAAGGGAGA | TTCTACATCCCATTGGAGATAAGTC | [46] |
agrD1 | TTTGCTAGCTCATTGGCACTT | GATTGCTGATTTCTTTGGGTACTT | Primer3 software |