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. 2019 Jul 19;17(7):e3000382. doi: 10.1371/journal.pbio.3000382

Fig 2. Lats1/2 deletion initiates EMT transition of pancreatic progenitors.

Fig 2

(A–J) Confocal images of VIM, CDH1, TAGLN, KRT19, and ACTA2 immunostains in WT and Lats1/2PanKO pancreata at (A–C, G) E11.5, (D, H) E12.5, or (E, F, I, J) E14.5 (n = 3 embryos per stage per genotype). “*” denotes absence of protein expression within pancreas (outlined in white). (G) Arrowhead indicates mosaic ACTA2 expression in pancreas progenitors at E11.5. Nuclei were counterstained with DAPI (blue). Scale = 25 μm. (K) Schematic illustrating partial EMT following Lats1/2 deletion, with acquisition of mesenchymal proteins including VIM, ACTA2, and TAGLN, normally absent from WT pancreas epithelium, and persistent CDH1. ACTA2, alpha smooth muscle actin; CDH1, E-cadherin; E, embryonic day; EMT, epithelial-mesenchymal transition; KRT19, cytokeratin 19; TAGLN, transgelin; VIM, vimentin; WT, wild type.