Table 3. Scoring of transgene expression at the different implant locations.
Rat 1 | Rat 2 | Rat 3 | Rat 4 | Sum of scores | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
i.m. | s.c. | ort. | i.m. | s.c. | ort. | i.m. | s.c. | ort. | i.m. | s.c. | ort. | i.m. | s.c. | ort. | |
pGFP 100 μg/ml | 2 | 0 | 1 | 2 | 0 | 3 | 2 | 1 | 3 | 0 | 1 | 1 | 6 | 2 | 8 |
pGFP 10 μg/ml | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | |||||
pGFP 1 μg/ml | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||||
pGFP + fibroblasts | 2 | 2 | 1 | 1 | 0 | 1 | 2 | 0 | 5 | 4 | |||||
pGFP + MSCs | 1 | 1 | 3 | 0 | 3 | 1 | 2 | 3 | 9 | 5 | |||||
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Each implant was given a score (0, 1, 2 or 3) for the amount of GFP expression (as detailed in the M&M section). The sum of the total scores per group is depicted. i.m.: intramuscular; s.c.: subcutaneous; ort.: orthotopic.