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. 2019 Apr 14;52(4):e12614. doi: 10.1111/cpr.12614

Figure 4.

Figure 4

Hsa‐miR‐132‐3p regulated the expression of Sox5 in BC. (A and B) qPCR analysis of candidate mRNA targets of hsa‐miR‐132‐3p in 5637 and EJ‐M3 cells transfected with the miR‐132 inhibitor and control miRNAs (inhibitor NC). GAPDH was used as the control. (C and D) qPCR and Western blot analyses of Sox5 expression in 5637 and EJ‐M3 cells transfected with the miR‐132 inhibitor and control miRNAs (inhibitor NC). GAPDH was used as the control. (E) Luciferase reporter assay for the binding of hsa‐miR‐132‐3p to predict binding sites on Sox5. Each experiment was repeated a minimum of three times. The symbol * denotes a significant difference (P < 0.05), while ** represents a highly significant difference (P < 0.01) in a two‐tailed Student's t test