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. 2019 Apr 14;52(4):e12614. doi: 10.1111/cpr.12614

Figure 5.

Figure 5

Hsa‐miR‐132‐3p plays a role in regulating Sox5 expression in BC. (A) qPCR analysis of Sox5 expression in BC (n = 23) and adjacent normal tissues (n = 32). GAPDH was used as the control. (B and C) qPCR analysis of the knockdown efficiency of siRNAs against Sox5 (siSox5) in 5637 and EJ‐M3 cells. GAPDH was used as the control. (D) Western blot analysis of the knockdown efficiency of siRNAs against Sox5 in 5637 and EJ‐M3 cells. GAPDH was used as the control. (E and F) CCK8 assay for the viability of 5637 and EJ‐M3 cells transfected with the miR‐132 inhibitor, miR‐132 inhibitor + siSox5 and control siRNAs (siNC). (G) EdU assay for the proliferation of 5637 and EJ‐M3 cells transfected with the miR‐132 inhibitor, miR‐132 inhibitor + siSox5 and control siRNAs (siNC). (H) Transwell assay for the migration potential of 5637 and EJ‐M3 cells transfected with the miR‐132 inhibitor, miR‐132 inhibitor + siSox5 and control siRNAs (siNC). Each experiment was repeated a minimum of three times. The symbol * denotes a significant difference (P < 0.05), while ** represents a highly significant difference (P < 0.01) in a two‐tailed Student's t test