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PCR primers sequences
| Gene name | Sequence 5′→3′ |
|---|---|
| RT‐PCR primers | |
| OCT4 | |
| Sense | CTGAAGCAGAAGAGGATCAC |
| Antisense | GACCACATCCTTCTCGAGCC |
| ISL1 | |
| Sense | GCGGAGTGTAATCAGTATTTGGA |
| Antisense | GCATTTGATCCCGTACAACCT |
| ESR1 | |
| Sense | TGATGAAAGGTGGGATACGAAA |
| Antisense | GGCTGTTCTTCTTAGAGCGTTTG |
| GAPDH | |
| Sense | CCATGTTCGTCATGGGTGTGA |
| Antisense | CATGGACTGTGGTCATGAGT |
| ChIP primers | |
| DNMT1 (OCT4) | |
| Sense | CCCCACACACTGGGTATAGAA |
| Antisense | CGAGGCATTCATTCATTCATT |
| DNMT1 (ERα) | |
| Sense | CAAGCCATCCTCCCACCTCAG |
| Antisense | CCAGCCTGAGCAACATAGGGATAC |
