Figure 5.

TRERNA1 promotes the ability of SNAI1 to treat HCC metastasis. A, Cell migration assays were performed using transwell membranes. The average number of cells exhibiting migration from three random microscopic fields is presented in the histogram. B, Cell invasion assays were performed using Matrigel‐coated transwell membranes. The average number of cells exhibiting invasion from three random microscopic fields is presented in the histogram. C and D, A wound‐healing assay in SNAI1‐overexpressing HepG2 cells and in SNAI1‐depleted HepG2.215 cells. The scratch was measured 0, 24 and 48 h later. E, Cell migration assays using transwells in HepG2 cells treated with the ctrl, SNAI, SNAI + TRERNA1, or SNAI + siTRERNA1. The average number of cells exhibiting migration from three random microscopic fields was presented in the histogram. F, Cell migration assays using transwells in HepG2.215 cells treated with the ctrl, siSNAI1, siSNAI + siTRERNA1, or siSNAI1 + TRERNA1. The average number of cells exhibiting migration from three random microscopic fields is presented in the histogram. The results are presented as the mean ± SD; n = 3. *P < 0.05, **P < 0.01 (Student's t test)