Figure 8.

G‐Rb3 inhibits cisplatin‐induced apoptosis through AMPK signal pathway in cultured HEK293 cells. Effects of G‐Rb3 against cisplatin‐induced cytotoxicity by regulating apoptosis. The protein expressions of Bcl‐2, Bax, Bcl‐XL, Atg5, Atg3, AMPK and caspase 3 were measured by Western blot analysis with specific primary antibodies, and GAPDH protein level was used as a loading normal (A). Effects of G‐Rb3 on cisplatin‐induced apoptosis in HEK293 cells via Hoechst 33258 staining. For Hoechst 33258 staining, yellow arrows indicate apoptotic cells (B). The cell viability was assessed by MTT assay. HEK293 were treated with cisplatin (20 µm), compound C, cisplatin plus compound C, and cisplatin plus compound C and G‐Rb3 (C). Quantification of Atg5, AMPK, caspase 3, Bcl‐2, Bax, Bcl‐XL and Atg3 relative protein expressions was performed by densitometric analysis (D‐G). All data were expressed as mean ± SD *P < 0.05 or **P < 0.01 comparing with normal group. ^# P < 0.05 or ^## P < 0.01 comparing with cisplatin group