Figure 9.

G‐Rb3 inhibits apoptosis via inhibition of autophagy in cultured HEK293 cells. Effects of G‐Rb3 against cisplatin‐induced cytotoxicity by regulating autophagy. The protein expressions of Atg3, Atg5, mTOR and caspase 3 were measured by Western blot analysis with specific primary antibodies, and GAPDH protein level was used as a loading normal (A). The cell viability was assessed by MTT assay. HEK293 cells were treated with cisplatin (20 µmol/L), Ram (200 nmol/L), cisplatin plus Ram, and cisplatin plus Ram and G‐Rb3 (1 µmol/L), respectively. Effects of G‐Rb3 on cisplatin‐induced apoptosis in HEK293 cells (B). Effects of G‐Rb3 on cisplatin‐induced apoptosis in HEK293 cells via Hoechst 33258 staining, yellow arrows indicate apoptotic cells (C). Quantification of Atg5, Atg3, caspase 3 and mTOR relative protein expressions was performed by densitometric analysis (D and E). All data were expressed as mean ± SD *P < 0.05 or **P < 0.01 comparing with normal group. ^# P < 0.05 or ^## P < 0.01 comparing with cisplatin group