Figure 1.

Six2 expression is significantly upregulated in NSCLC tissues and negatively correlated with the OS of lung cancer patients. A, Six2 mRNA was detected in NSCLC and normal tissues via qRT‐PCR assay. B, C, Six2 protein level was examined in NSCLC and normal tissues via Western blot and immunohistochemistry assays. D, E, The correlation between six2 expression and the first progression, and OS of lung cancer patients was analysed via KM plotter assay. F, G, Six2 mRNA (F) and protein (G) level were determined in NSCLC cells and normal lung epithelial cells. Data were presented as mean ± SD; *P < 0.05, **P < 0.01 vs normal tissues or BEAS‐2B