Figure 3.

Knockdown of six2 attenuated NSCLC cell stemness. A, B, Knockdown efficiency of six2 was measured in NSCLC cells with six2 stable knockdown or not via qRT‐PCR (A) and Western blot (B) assay. C, D, Expression of stemness markers (ALDH1 and Nanog) was detected in the cells depicted in (A) via qRT‐PCR and Western blot assay. E, ALDH1 activity was examined in cells depicted in (A). F, G, Spheroid size (F) and number (G) of NSCLC cells with or without six2 knockdown were determined. H, Images of tumours harvested when serially diluted NSCLC cells with or without six2 knockdown were planted. Data were presented as mean ± SD; *P < 0.05, **P < 0.01 vs A549 or NCI‐H1299