Figure 6.

Six2 promotes NSCLC cell stemness and migration partly dependent on E‐cadherin expression. A, B, The mRNA levels of stemness markers (ALDH1 and Nanog) were detected in NSCLC cells with six2 knockdown plus E‐cadherin knockdown or not. C, D, The protein levels of stemness markers (ALDH1 and Nanog) and E‐cadherin were examined in NSCLC cells depicted in (A). E, ALDH1 activity was measured in the cells depicted in (A). F, G, Spheroids size and number was detected in the cells depicted in (A). H, Migration ability was determined in the cells depicted in (A). I, Images of tumours harvested when NSCLC cells depicted in (A) at the density of 1 × 10⁴ cells were planted. Data were presented as mean ± SD; *P < 0.05, **P < 0.01 vs A549 or NCI‐H1299