Figure 7.

Knockdown of six2 attenuated cisplatin resistance of A549‐DDP cells. A, B, Expression of stemness markers (ALDH1 and Nanog) was detected in A549 and A549‐DDP cells via qRT‐PCR (A) and Western blot (B) assay. C, ALDH1 activity was measured in A549 and A549‐DDP cells. D, E, Spheroid size and number were examined in A549 and A549‐DDP cells. F, G, Expression of drug resistance genes (ABCG2 and MDR1) was determined in A549 cells, A549 spheroids and A549‐DDP cells. H, Six2 expression was detected in A549 and A549‐DDP cells. I, J, A549‐DDP cells with or without six2 knockdown were treated with cisplatin, and followed by cell viability (I) and cell apoptosis (J) assay. K, Expression of apoptosis executors (cleaved caspase 3 and cleaved PARP) was examined in the cells depicted in (I). Data were presented as mean ± SD; *P < 0.05, **P < 0.01 vs A549‐DDP or A549