Table 2.

Primers used in this study

Gene	Forward primer	Reverse primer	Size (bp)
Ccnd1	GCGCAGACCTTCGTTGCCCT	GCCGTTGGCGCTTCCCAGAT	123
Ccnd2	AACACCGATGTGGATTGCC	GGAGAGAGCGGATTGGACG	211
Ccnd3	ATTGGGAGGTGCTGGTCTTG	TGTGGCAATCATGGATGGGG	200
Ccne1	ACTTCTGTACCCACACGCTG	TTGCTCGCATTTTAGGCTGC	194
Cdk2	GCTCACTGGCATTCCTCTTC	ACCCATCTGCGTTGATAAGC	134
Cdk4	AGTGACCCTGGTGTTTGAGC	GCAGTTGGCATGAAGGAAAT	142
Cdk6	TCGTGGAAGTTCAGATGTCG	TTGGTTGAGGGGATTTTGAG	128
Col X	ATCCCCGGCCCAGCTGGAAT	GGGAGGCCCCTCTCACCTGG	179
Runx2	TCAGAACCCACGGCCCTCCC	GACAGCGGCGTGGTGGAGTG	177
Alpl	GGAAGGGGGCAGGATTGAC	GGTGTACCCGCCAAAGGTAA	175
Notch1	AACCGTAGCTCCTGAGAGCA	AGAGTCTGATCGTGCCCACT	116
Notch2	TCGCTTCCAGTGTCTGTGTC	ACACTTTGCCCCATTCAGAC	100
Shh	GTGATCCTTGCTTCCTCGCT	TGTCGGGGTTGTAATTGGGG	223
Smo	GTTCGGACAGACAACCCCAA	GATTCGAGTTCCGCCAGTCA	190
Gli1	CTGAGCCTTATGGAGCTAGA	AATGTTCAAGACGAGGACAC	207
Gli2	GCACCACCCCCTCAGACTAT	AGAGTGGGGAGATGGACAGC	207
Gli3	ACCATACGTCTGTGAGCACG	ATGTTTCCGGAGGGAGCTTG	160
Foxa1	CTTTCAAGCGCAGCTATCCT	TCGCTCAGTGTGAGCATCTT	103
Foxa2	TCATGTCGTCAGAGCAGCAG	CCCCTGGTAGTAGGAGGTGT	200
Foxa3	GGGCTCGGTGAAGATGGAG	GTCATGTAGGAGTTGAGGGGG	120
GAPDH	GAAGGGTGGCGCCAAGAGGG	GGGGGCCAAGCAGTTGGTGG	142