Figure 2.

GDF11 inhibits adipogenic differentiation of 3T3‐L1 pre‐adipocytes. A, MTS assay of 3T3‐L1 pre‐adipocytes treated with different concentrations of rGDF11. B, Oil Red O staining of 3T3‐L1 pre‐adipocytes 7 d after adipogenic differentiation. Scale bar, 25 μm. C, Quantification of lipid accumulation in of 3T3‐L1 pre‐adipocytes supplemented with rGDF11. Triglyceride content, measured at 500 nm after extracting Oil Red O, was diminished in 50 and 100 ng mL⁻¹ rGDF11‐treated groups. D, qRT‐PCR results. The relative mRNA expressions of adipocyte‐specific molecular markers Pparg, Cebpa, Lpl, Plin1, Cd36 and Adipoq were analysed on Day 3 after differentiation. E, qRT‐PCR results. The relative mRNA expressions of adipocyte‐specific molecular markers Pparg, Cebpa, Lpl, Plin1, Cd36 and Adipoq were analysed on Day 5 after the differentiation. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001. Results were shown as mean ± SEM, ANOVA