Figure 6.

JIB‐04, a H3K36me3 demethylase, restores the H3K36me3 level and promotes imatinib (IM) sensitivity in TF1‐BAR cells. A, Western blot assay of H3K36me3 and H3K9me3 expression changes in TF1‐BAR cells after treatment with different doses of JIB‐04 for 72 h. B, C, CCK‐8 assay and IC₅₀ curves of the effects of JIB‐04 alone or in combination with IM on TF1‐BAR cells. D, E, Flow cytometric assay of the apoptosis of TF1‐BAR cells after JIB‐04 (1 μmol/L) and/or IM treatment for 72 h. F, G, Flow cytometric assay of the percentage of CD34⁺CD38⁻ stem cells of TF1‐BAR cells after JIB‐04 (1 μmol/L) and/or IM treatment for 72 h. H, Colony formation assay of the effects of JIB‐04 (1 μmol/L) and/or IM treatment on the quantity of stem cells in TF1‐BAR cells. Bars in the graphs represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, by ANOVA