Figure 6.

MFI2‐AS1 siRNA affects cell cycle distribution and apoptosis. A, Cell cycle analysis was performed using flow cytometry. Cells were transfected with miR‐574‐5p mimics for 48 h. B, Hoechst staining for cells in response to miR‐574‐5p mimics. C, the predicated binds sites of miR‐574‐5p to MYCBP 3′‐UTR. D dual‐luciferase reporter assay. E, the western blot analysis of MYCBP protein in cells transfected with miR‐574‐5p mimics. * and ** note P < 0.05 and P < 0.01 vs NC, respectively. MUT, mutant 3′UTR of_MYCBP; NC, negative control; WT, wild type of 3′UTR