Table 1.
The PCR primers used in this study
| Gene name | Primers | Sequence (5′‐3′) |
|---|---|---|
| GAPDH | Forward | TGTTCGTCATGGGTGTGAAC |
| Reverse | ATGGCATGGACTGTGGTCAT | |
| MFI2‐AS1 | Forward | TACATACAGTGACCCAAAGAGCA |
| Reverse | CAGTGCTTCTGAACGCCTCTT | |
| U6 | Forward | CTCGCTTCGGCAGCACA |
| Reverse | AACGCTTCACGAATTTGCGT | |
| miR‐574‐5p | RT |
CTCAACTGGTGTCGTGGA GTCGGCAATTCAG TTGAGAC ACACTCA |
| miR‐574‐5p | All R | CTCAACTGGTGTCGTGGA |
| miR‐574‐5p | Forward | ACACTCCAGCTGGG TGAGTGTGTGTGTGTGA |
| miR‐19‐3p | RT |
CTCAACTGGTGTCGTGGA GTCGGCAATTCAG TTGAGAC TCAGTTT |
| miR‐19‐3p | All R | CTCAACTGGTGTCGTGGA |
| miR‐19‐3p | Forward | ACACTCCAGCTGGG TGTGCAAATCTATGCA AAA |
| miR‐218‐5p | RT |
CTCAACTGGTGTCGTGGA GTCGGCAATTCAG TTGAGAC ACATGGT |
| miR‐218‐5p | All R | CTCAACTGGTGTCGTGGA |
| miR‐218‐5p | Forward | ACACTCCAGCTGGG TTGTGCTTGATCTA ACC |
| miR‐375 | RT |
CTCAACTGGTGTCGTGGA GTCGGCAATTCAG TTGAGAC TCACGCG |
| miR‐375 | All R | CTCAACTGGTGTCGTGGA |
| miR‐375 | Forward | ACACTCCAGCTGGG TTTGTTCGTTCGGCTCGC |
| miR‐130‐3p | RT |
CTCAACTGGTGTCGTGGA GTCGGCAATTCAG TTGAGAC ATGCCCT |
| miR‐130‐3p | All R | CTCAACTGGTGTCGTGGA |
| miR‐130‐3p | Forward | ACACTCCAGCTGGG CAGTGCAATGTTAAA AGG |