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. Author manuscript; available in PMC: 2019 Jul 31.
Published in final edited form as: Virology. 2018 Feb 12;518:14–24. doi: 10.1016/j.virol.2018.02.005

Fig. 4.

Fig. 4.

Validation results for BevuMV1-VDH66156. Positions of DNA markers are labeled at left (bp). RNA or DNA extracts were obtained from leaves of sugar beet strain VDH66156. (A) RNA extract was used for RT–PCR amplification using three different primer pairs specific for BevuMV1 (V1–V3). (B) RNA extract was used for PCR amplification (no RT step) using two different primer pairs specific for BevuMV1 (V1 and V3). At right is an RT-PCR control using one primer pair specific for BevuMV1 (V1). (C) DNA extract was used for PCR amplification (no RT step) using one primer pair specific for BevuMV1 (V1). At right are PCR controls using two primer pairs specific for B. vulgaris chloroplast DNA (C1 and C2) and two primer pairs specific for B. vulgaris mitochondrial DNA (M1 and M2).