Figure 1. Unsupervised ordering of the hGFAP-GFP-derived cells reveals developmental hierarchy.

(A) Scheme for analysis of hGFAP-GFP⁺ cells using scRNA-seq from neonatal cortices (n=5 mice).

(B) t-SNE analysis of hGFAP-GFP⁺ cell clusters.

(C) Heatmap of hGFAP-GFP⁺ cells ordered as t-SNE (n = 815). Columns, individual cells; rows, genes.

(D) The proportions of distinct clusters among total hGFAP-GFP⁺ cells.

(E) Dot plot of levels of selected marker genes in subpopulations.

(F-G) t-SNE plots of F) astrocyte (Astro), radial glia (RG), and iGC and G) marker genes.

(H) Comparison of astrocyte and iGC clusters with adult astrocyte populations.

(I-J) t-SNE plot of I) OPC and pri-OPC cells and J) marker genes.

(K) Pseudo-time ordering of pri-OPCs and OPCs in hGFAP-GFP⁺ dataset. Red line, the predicted trajectory.

(L) Predicted lineage trajectories from RG-like cells in hGFAP-GFP⁺ cells.

See also Figure S1 and Table S2.