Figure 1.
Formation of bACs aggregates in spinner flask. The effect of agitation rate (40, 50 and 60 rpm) on the (A) bACs aggregation and (B) kinetics of bACs aggregation over 24 h of culture. The effect of cell inoculum density (2, 4 and 8 × 105 cells/mL) on the (C) bACs aggregation and (D) kinetics of bACs aggregation over 24 h of culture. (E) Low‐magnification (2000×) SEM analyses of bACs aggregation after 1, 3 and 5 d of culture. Scale bar = 10 μm. The size distribution (F) and the average size distribution (G, H) of bACs aggregates after 1, 3 and 5 d of culture (n = 500‐1000 per time point)