Figure 2.
Formation of rMSCs aggregates in spinner flask. The effect of agitation rate (40, 45 and 50 rpm) on the (A) rMSCs aggregation and (B) kinetics of rMSCs aggregation over 24 h of culture. The effect of cell inoculum density (2, 4 and 8 × 105 cells/mL) on the (C) rMSCs aggregation and (D) kinetics of rMSCs aggregation over 24 h of culture. (E) Low‐magnification (2000×) analyses of rMSCs aggregation after 1, 3 and 5 d of culture. Scale bar = 10 μm. The size distribution (F) and the average size distribution (G, H) of rMSCs aggregates (n = 500‐1000 per time point)