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Diagram of how to use FIJI to enumerate cells in highly packed conditions. (a) → (b): transfer color images to 16-bit gray scale images. (b) → (c): tune threshold to remove most of the out-of-focus cells and keep most of the in-focus cells. (c) → (d): run watershed in FIJI to distinguish the highly packed cells. Run Analyze Particles function to count the cells in image (d).
