Figure 1:

Construction of the human Respiratory Microbial Gene Catalogue (RMGC). Genome assembly was performed for each sample with ≥650 Mb of data. For samples with <650 Mb of data, data from the same respiratory site (NP, OP, or lung) were mixed and assembled. Gene predictions were conducted for all assembled contigs with ≥500 bp and respiratory bacterial genomes in the Integrated Microbial Genomes and Microbiomes (IMG) database. Genes with ≥100 bp were retained. Respiratory gene sets from the Human Microbiome Project (HMP) and Pathosystems Resource Integration Center (PATRIC) were combined to construct the non-redundant RMGC containing 2,245,343 genes.