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. Author manuscript; available in PMC: 2019 Jul 31.
Published in final edited form as: ACS Infect Dis. 2018 Mar 1;4(5):806–814. doi: 10.1021/acsinfecdis.7b00235

Figure 4.

Figure 4

PPMOs have low cytotoxicity and are efficacious in a mouse model of pneumonia. (A) Human CF bronchial epithelial cells (CFHBEs) were seeded onto 96-well tissue cultures plates, stained with resazurin, and incubated for 24 h. Triton X-100, a detergent, was used as a positive cytotoxicity control. (B) CYBB mice, deficient in the phagocyte oxidase, were infected with 6 × 105 CFU of B. multivorans SH-2 by intranasal instillation of 50 μL coadministered with 50 μg of PPMO (2.5 mg/kg). (C) A second group received a second dose at 6 h postinfection in 25 μL (total dose 100 μg or 5 mg/kg). Mice were euthanized at 24 h, and lungs were homogenized for CFU enumeration (n = 5–7). Significance was determined with a Mann–Whitney U test; *p ≤ 0.05, **p ≤ 0.01.