Figure 2.

(A) Schematic of the click chemistry coupling between two modified guanines in the top G-tetrad and two duplex DNA handles for mechanical unfolding experiments. Dotted arrows depict unfolding directions. (B) Rupture force histograms of the constructs unfolded through the Top, Middle, and Bottom G-tetrads. The histograms are fitted with the Dudko equation (solid, see SI) without (top) and with (bottom)100 nM L2H2–6OTD in a 10 m M Tris buffer (pH 7.4, 100 mM KCl). Residues of each fitting are shown to the top. The black dotted lines depict average values