Table 1.

Rupture force (F_unfold, pN), unfolding rate constant (k_unfod, s⁻¹), unfolding energy barrier (ΔG^†_unfold, kcal/mol)’ and distance from the folded to the transition state (x^†) of telomeric DNA G-quadruplex via different unfolding geometries without and with 100 nM L2H2–6OTD monomer

Variable	Ligand	Top	Middle	Bottom	L2–3'	5’-L2	L1–3'	5’-L3	L1-L3
Funfold (pN)	No	33±2.4	21±0.5	32±1.9	44±1.6	42±0.9	34±0.9	34±1.3	46±0.5
	Yes	39.3±1.1	32.2±1.8	36.5±1.9	40.4±1.5	32.7±0.5	34.3±1.8	34.7±1.0	38±1.2
kunfold (s−1)	No	0.019±0.001	0.053±0.005	0.018±0.001	0.0048*	0.0055*	0.0111*	0.015*	0.0009*
	Yes	0.013±0.003	0.029±0.004	0.013±0.002	0.007±0.003	0.016±0.005	0.015±0.004	0.019±0.001	0.006±0.001
ΔG†unfold, (kcal/mol)	No	14.8±0.8	15.3±0.2	12.9±0.7	19±3.0*	17.5±0.4*	17.1±2.0*	21*	21±1.0*
	Yes	19.8±0.3	20.6±1.3	19.7±0.9	14.8±1.0	14.3±0.7	15.1±1.2	15.6±0.6	13.3±1.2
x† (nm)	No	0.10±0.01	0.12±0.01	0.11±0.01	0.10*	0.10*	0.11*	0.08*	0.14*
	Yes	0.09±0.01	0.08±0.01	0.09±0.01	0.12±0.02	0.11±0.02	0.11±0.01	0.09±0.01	0.14±0.04

^*:data taken from reference^[2].