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. 2019 Jan 24;104(8):1608–1616. doi: 10.3324/haematol.2018.207837

Figure 2.

Figure 2.

Zeb2-mediated T-cell differentiation delay is independent of the thymic mirco-environment. (A,B) Flow cytometric analysis of 1- and 2-week in vitro differentiation cultures of E13.5 fetal liver hematopoietic progenitors on OP9-DL1 feeders. Percentages of DN1 (CD4/8− and CD44+, CD25−), DN2 (CD4/8− and CD44+, CD25+), DN3 (CD4/8−, CD44−, CD25+) and post-DN3 (sCD3 or CD8+) populations show a significant delay in differentiation upon Zeb2 overexpression (A) and the presence of a cell population with intermediate CD25 expression even after 4 weeks of co-culture, as exemplified by a representative dot plot (B). (C) Lowering the concentration of recombinant interleukin 7 (IL7) in OP9-DL1 co-cultures partially rescues the delay in T-cell differentiation of R26-Zeb2tg/tg fetal liver hematopoietic progenitors. Dot plots of representative cultures are shown as are the percentages of T-cell populations following addition of 5 ng/mL, 1 ng/mL and 0.2 ng/mL recombinant IL7. *P<0.05, **P<0.01, ***P<0.001 (vs. control).