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. 2019 Feb 21;104(8):1597–1607. doi: 10.3324/haematol.2018.192773

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Figure 1. — Upregulation of RUNX1, KLF4 and P57 in t(8;21) leukemia cells by sodium phenylbutyrate (PB) treatment. (A) Relative expressions of RUNX1, KLF4 and P57 mRNA were measured by quantitative real-time polymerase chain reaction in Kasumi-1 cells after treatment with different doses of PB for the time (hours, h) indicated. The gene transcript levels were normalized to those of GAPDH and set to 1 in the control group. (B) Western blot analysis of RUNX1, KLF4 and P57 protein expressions in Kasumi-1 cells after PB treatment. β-actin and H3 were used as internal loading controls.