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. 2019 Feb 7;104(8):1648–1660. doi: 10.3324/haematol.2018.208363

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Figure 3. — Enhanced re-epithelialization and angiogenesis occur at the early phase of wound healing in the absence of GPVI and CLEC-2. (A) Hematoxylin & Eosin staining at day 3 post-injury (n=6-9). Dotted line indicates hyperplastic coverages. Black arrow points to wound edge. Red arrow indicates gap between epithelial tongues. S: scab; G: granulation tissue. Scale bar=500 μm. (B) Measurement of re-epithelialization. (C) Measurement of wound contraction. (D) Quantification of granulation tissue area. (E) Detection of endothelial cells (CD31⁺ cells; green) in wound area at day 3 post injury. Hoechst counterstains nuclei (blue). Scale bar=50 μm. (F) Quantification of CD31⁺ area within the wound at day 3 post injury (n=5-6). Graphs are presented as mean±Standard Error of Mean and analyzed by one-way ANOVA with Bonferroni’s multiple comparison test. *P<0.05.