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a) LOD of ddPCR measured with spike samples of nucleic acids containing KRAS G12D mutation in various allele frequency. Error bars correspond to standard deviation (n=5). b) LOD of ddPCR measured with spike samples of nEVs derived from Panc-1 cell at various allele frequency. Error bars corresponds to standard deviation (n=5). c) summary of 3 patients with metastatic pancreatic adenocarcinoma (PAAC) d) ddPCR plots for KRAS mutations detection in the 3 respective PAAC patients.
