Fig. 1.

Cytopathology and growth properties of PEDV G2b isolate Aram. (A) CPE formation in Vero cells infected with Aram-P5 virus. PEDV-specific CPE was observed daily, and cells were photographed at 24 hpi using an inverted microscope at a magnification of 200× (left panels). For immunostaining, infected cells were fixed at 24 hpi and incubated with MAb against the N protein, followed by incubation with Alexa green-conjugated goat anti-mouse secondary antibody. The cells were then examined under a fluorescence microscope at 200× magnification (left panels). (B) One-step growth kinetics for Aram-P5. At the indicated time points post-infection, culture supernatants were harvested from Aram-P5-infected Vero cells, and virus titers were determined.

CPE, cytopathic effects; PEDV, porcine epidemic diarrhea virus; DAPI, 4′,6-diamidino-2-phenylindole; TCID, tissue culture infectious dose.