Fig. 2.

Sequence alignments of the N-terminal region of the S protein (A) and the C-terminal region of ORF3 (B) of global PEDV strains. Genetic subgroups of PEDV are indicated in parenthesis on the left. The dashes (–) indicate deleted sequences. Potential N-glycosylation sites in the S protein predicted by the NetNGlyc 1.0 server (http://www.cbs.dtu.dk/services/NetNGlyc/) are shown in boldface type. The S insertions-deletion genetic signature of G2 epidemic strains, that includes two discontinuous 4-aa and 1-aa insertions at positions 55/56 and 135/136 and one 2-aa DEL at positions 160 and 161 within S compared to the prototype CV777 strain, is shown in solid boxes. The Aram strain-specific DELs in S and ORFs are shaded. An alternative TAA stop codon (boldface) and a corresponding premature termination (asterisk) in nucleotide (top panel) and deduced amino acid (bottom panel) sequences of ORF3 are also indicated.

ORF, open reading frame; PEDV, porcine epidemic diarrhea virus; DEL, deletion.