Fig. 6.

Clinical significance scores and virus shedding in piglets inoculated with PEDV Aram-P5, -P29-CA, or mock. (A) Clinical significance scores were measured as described in the Materials and Methods section. (B) PEDV titers in rectal swap samples at each time point were determined by a quantitative real-time reverse transcription polymerase chain reaction. The virus titers (log₁₀ tissue culture infectious dose₅₀/mL) are the mean virus titers from all pigs and error bars represent the mean ± standard deviation of the mean. The p values were calculated by comparing the parental (Aram-P5) virus- and cold-adapted (P29-CA) virus-inoculated groups using Student's t-test.

PEDV, porcine epidemic diarrhea virus.

^*p < 0.001.