Fig. 8.

Clinical significance scores, survival rates, and virus shedding in piglets from three experimental groups. Pregnant sows were primed/boosted orally with a live Aram-P29-CA vaccine at 2-week intervals pre-farrowing and their nursing piglets were challenged with virulent G2b PEDV at 4 days of age (A) Clinical significance scores were measured as described in the Materials and Methods section. (B) Survival rate of piglets from vaccinated (group 1), challenge-control (group 2), and negative-control (group 3) sows through 7 DPC. (C) PEDV titers in rectal swap samples at each time point were determined by quantitative real-time reverse transcription polymerase chain reaction analysis. The virus titers (log₁₀ tissue culture infectious dose₅₀/mL) are the mean virus titers from all pigs and error bars represent the mean ± standard deviation of the mean. The p values were calculated by comparing the data from the vaccinated and unvaccinated sow groups after challenge using Student's t-test.

PEDV, porcine epidemic diarrhea virus; DPC, days post-challenge.

^*p = 0.001 to 0.05; ^† p < 0.001.