Fig. 9.

PEDV-specific neutralizing antibody responses in serum (A) and colostrum (B) samples of sows and sera (C) of their corresponding litters from three experimental groups. Pregnant sows were primed/boosted orally with a live Aram-P29-CA vaccine at 4 and 2 weeks pre-farrowing and their nursing piglets were challenged with virulent G2b PEDV at 4 days of age. The samples were collected at the indicated time points and were tested by virus neutralization assay using Aram-P5. Neutralizing antibody titers for individual samples were presented as a log₂ scale. Values are representative of the mean from three independent experiments in duplicate and error bars denote the mean ± standard deviation of the mean. The p values were calculated by comparing the data from the vaccinated and unvaccinated sow groups after challenge using Student's t-test.

^*p = 0.001 to 0.05; ^† p < 0.001.