Fig. 1. The recombinant adenoviruses rAd-Cap-InvC, rAd-Cap and rAd infected PK-15 cells. The expression of enhanced green fluorescent protein could be observed under inverted fluorescence microscope in rAd (A, B), rAd-Cap (C, D) and rAd-Cap-InvC (E, F) infected PK-15 cells. (G) Western blot of Cap-InvC and Cap in the infected PK-15 cells. Lanes 1–3: rAd-Cap, rAd-Cap-InvC, and rAd infected cells. GAPDH was used as an internal reference.
Cap, protein capsid; InvC, C-terminal domain of Yersinia pseudotuberculosis invasin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PK, pig kidney.