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Metabolic challenge. Female mice were given a high fat high sugar diet (HFHSD) at 11 months (A) and 12 months of age (B). After 3 days, either a glucose tolerance test (GTT) was performed at 11 months of age (A) or mice were placed in metabolic cages for indirect calorimetry for 3 days (B). Mice were allowed to acclimate to the new cage environment on first day (Day 4) of indirect calorimetry and Days 5 and 6 were used for data analysis.
