Figure 6.

Proteomics-based inference of A. suum intestinal proteins in different compartments. (A) Anatomy of model intestinal nematode species Ascaris suum (transverse section). (B) Protein sets detected by MS/MS proteomics from samples harvested from adult A. suum worms (left) have other protein sets strategically removed (center) to deduce final protein sets in different intestinal compartments (right). “Integral intestinal membrane” proteins are not labelled as “basal” because they may include some proteins from the apical intestinal membrane as well. *Proteins annotated with “cellular compartment” Gene Ontology terms for endoplasmic reticulum, mitochondria, Golgi apparatus, and nucleus were removed to reduce contamination from proteins embedded in these organelles rather than the external cellular membrane. **Only proteins annotated with predicted classical or nonclassical secretion signals were included since these are better candidates for proteins that are transported to the membrane.